Creative Biogene offers biochemical assays for a wide range of HMT enzyme targets, including DOT1, EZH1, EZH2, G9a, NSD1, NSD2, PRMT1, PRMT3, PRMT4, PRMT5, PRMT6, PRMT8, SET1B, SET7/9, SET8, MLL-1, SMYD3, SUV39H1, SUV39H2, and so on. HMTs assays in Creative Biogene utilize highly specific antibodies that recognize methyl groups. Generally, there are three steps in our assays. First, S-adenosylmethionine (SAM) is incubated with a sample containing assay buffer and purified recombinant methyltransferase enzyme. Second, acceptor beads, a primary antibody, and then donor beads are added. The third step is to read the Alpha-counts.
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